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KMID : 0360419840200020049
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Korean Journal of Pharmacology
1984 Volume.20 No. 2 p.49 ~ p.57
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Amylase Release from Pancreatic Slices of Rat Treated with Adrenergic Drugs
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Kim Kyeong-Hwan
Hong Sa-Suk
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Abstract
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The exocrine pancreatic secretion is controlled mainly by gastrointestinal hormones as well as cholinergic nerves. The adrenergic influence on exocrine pancreas is thought not to be important and the evidences supporting this contention are still contradictory. In an effort to elucidate the adrenergic influence on the exocrine pancreas, we have determined the amylase release from pancreatic slices of rats treated with adrenergic drugs. The albino rats of either sex, weighing 60?80 g, were decapitated and the uncinate pancreata were isolated and incubated in screw top vials containing 2 ml Krebs-Ringer bicarbonate buffer solution gassed with 95% Oa and 5% CO2. These vials were shaken continuously in a waterbath maintained at 37 °C, and enzyme release was stimulated with acetylcholine(10-5M), For chronic treatment methoxamine(an a-adrenergic agonist, 5 mg/kg), isoproterenol (a /3-adrenergic agonist, 10 mg/kg) and reserpine(0.5 mg/kg) along with cholecystokinin octapeptide(CCK-op, 2 pg/kg) were given i.p. in rats daily for 3, 5, 7, 9 or 12 days. For acute experiment these drugs were added directly to the incubation medium in a concentration of 10-5M except CCK -OP (10-´M) .
The results are summarized as follows.
1) The addition tsf methoxamine, isoproterenol or reserpine to the incubation medium containing pancreatic slices augmented the release of amylase induced by acetylcholine and among them the er ;sct of isoproterenol was most prominent.
2) Chronic treat :rent of methoxamine or reserpine caused enhancement of acetylcholine response in amylase release from pancreatic slice throughout the experimental period, but the amylase release was less than that of control by 12 days isoproterenol treatment.
3) In the pancrea : is slices obtained from 12 days treatment of CCK-OP, the amylae release responding to acetyicholine was enhanced.
By these finding ; is suggested that methoxamine, isoproterenol and reserpine had marked influence on the exocrine pancreatic functions in rats and that these effects are due to their inherent actions ray:ner than sympathetic nerve or adrenergic receptor function.
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